Primer Pair Tm Matching
Forward and reverse primer Tm values should agree within about 2 C so a single annealing temperature is near-optimal for both. A mismatched pair forces a compromise that leaves one primer non-specific and the other under-annealed.
This Concept is waiting for its first lesson!
Forward and reverse primer Tm values should agree within about 2 C so a single annealing temperature is near-optimal for both. A mismatched pair forces a compromise that leaves one primer non-specific and the other under-annealed.
Are you a teacher? Sign in to start contributing.
Sign In