Protease and Phosphatase Inhibition
Lysis releases proteases and phosphatases that degrade the target and strip phospho-epitopes within minutes, so broad-spectrum inhibitor cocktails plus serine-protease inhibitors are added fresh to ice-cold buffer. Phospho-specific work additionally requires sodium orthovanadate and fluoride or a dedicated phosphatase cocktail, since protease inhibitors alone do not protect phosphorylation state.
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Lysis releases proteases and phosphatases that degrade the target and strip phospho-epitopes within minutes, so broad-spectrum inhibitor cocktails plus serine-protease inhibitors are added fresh to ice-cold buffer. Phospho-specific work additionally requires sodium orthovanadate and fluoride or a dedicated phosphatase cocktail, since protease inhibitors alone do not protect phosphorylation state.
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