Protein Blocking of Nonspecific Binding
Normal serum from the secondary antibody host, BSA, or a casein-based reagent coats charged and hydrophobic tissue surfaces so immunoglobulin cannot stick nonspecifically. Fc receptors on macrophages and Fc-mediated background are addressed with the same or an Fc-specific block.
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Normal serum from the secondary antibody host, BSA, or a casein-based reagent coats charged and hydrophobic tissue surfaces so immunoglobulin cannot stick nonspecifically. Fc receptors on macrophages and Fc-mediated background are addressed with the same or an Fc-specific block.
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