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Protein Purification

Isolating a single protein species to near-homogeneity by exploiting differences in affinity, size, and charge, usually an affinity capture step followed by size-exclusion polishing. Crystallization demands both chemical purity and conformational homogeneity — a monodisperse sample matters more than a high yield.

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Isolating a single protein species to near-homogeneity by exploiting differences in affinity, size, and charge, usually an affinity capture step followed by size-exclusion polishing. Crystallization demands both chemical purity and conformational homogeneity — a monodisperse sample matters more than a high yield.

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