Proteolytic Epitope Retrieval
Proteases such as trypsin, pepsin or proteinase K digest cross-linked protein to expose the epitope, and are preferred for a minority of targets that heat destroys. Over-digestion shreds morphology and creates false negatives, so digestion time is titrated tightly.
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Proteases such as trypsin, pepsin or proteinase K digest cross-linked protein to expose the epitope, and are preferred for a minority of targets that heat destroys. Over-digestion shreds morphology and creates false negatives, so digestion time is titrated tightly.
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