qPCR Amplification Curve Phases
A real-time PCR trace passes through a baseline phase where product fluorescence is below detection, an exponential phase where product roughly doubles each cycle, a linear phase as reagents deplete, and a plateau where amplification stops. Only the exponential phase carries quantitative information about starting template.
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A real-time PCR trace passes through a baseline phase where product fluorescence is below detection, an exponential phase where product roughly doubles each cycle, a linear phase as reagents deplete, and a plateau where amplification stops. Only the exponential phase carries quantitative information about starting template.
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