qPCR Standard Curve
A standard curve plots Cq against log10 of template quantity for a dilution series of known concentration, fitted by linear regression. Its slope gives amplification efficiency, its intercept anchors absolute quantification, and its R-squared and residuals reveal pipetting error and inhibition at the extremes of the series.
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A standard curve plots Cq against log10 of template quantity for a dilution series of known concentration, fitted by linear regression. Its slope gives amplification efficiency, its intercept anchors absolute quantification, and its R-squared and residuals reveal pipetting error and inhibition at the extremes of the series.
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