Raman Excitation Wavelength Selection
Raman intensity scales roughly as the fourth power of the scattered frequency, so shorter-wavelength excitation gives more signal, but it also lands on more electronic absorptions and generates far more fluorescence. Practical work trades these off, with 532 nm for strong signal on non-fluorescing samples and 785 nm or 1064 nm to suppress fluorescence at the cost of intensity and detector sensitivity.
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Raman intensity scales roughly as the fourth power of the scattered frequency, so shorter-wavelength excitation gives more signal, but it also lands on more electronic absorptions and generates far more fluorescence. Practical work trades these off, with 532 nm for strong signal on non-fluorescing samples and 785 nm or 1064 nm to suppress fluorescence at the cost of intensity and detector sensitivity.
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