Reference Gene Selection and Validation
A reference gene is only valid if its expression is stable across the specific tissues, treatments, and time points of the experiment, which must be demonstrated with a stability measure such as geNorm M or NormFinder rather than assumed from tradition. Best practice normalises to the geometric mean of two or more validated reference genes, because a single gene's drift propagates directly into every fold change reported.
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A reference gene is only valid if its expression is stable across the specific tissues, treatments, and time points of the experiment, which must be demonstrated with a stability measure such as geNorm M or NormFinder rather than assumed from tradition. Best practice normalises to the geometric mean of two or more validated reference genes, because a single gene's drift propagates directly into every fold change reported.
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