Reversed-Phase HPLC Purification of Peptides
Crude peptide is resolved on a C18 or C4 stationary phase with a water/acetonitrile gradient containing 0.1% TFA as an ion-pairing agent, separating the target from deletion and adduct species that differ by a single residue. Analytical conditions are scouted first, then scaled to preparative columns with the gradient shallowed around the target.
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Crude peptide is resolved on a C18 or C4 stationary phase with a water/acetonitrile gradient containing 0.1% TFA as an ion-pairing agent, separating the target from deletion and adduct species that differ by a single residue. Analytical conditions are scouted first, then scaled to preparative columns with the gradient shallowed around the target.
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