SDS-PAGE Protein Separation
Denatured, SDS-coated polypeptides migrate through a discontinuous polyacrylamide gel where the stacking gel concentrates them into a sharp front and the resolving gel sieves them so mobility varies with the logarithm of molecular weight. Separation quality depends on the gel percentage, run voltage, and keeping the sample fully denatured and reduced.
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Denatured, SDS-coated polypeptides migrate through a discontinuous polyacrylamide gel where the stacking gel concentrates them into a sharp front and the resolving gel sieves them so mobility varies with the logarithm of molecular weight. Separation quality depends on the gel percentage, run voltage, and keeping the sample fully denatured and reduced.
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