Single-Particle Cryo-Electron Microscopy
Thousands of individual molecules vitrified in random orientations are imaged and computationally averaged into a 3D Coulomb-potential map, with phases recovered directly from the images so there is no phase problem. It complements crystallography by handling large complexes and membrane proteins that resist crystallization, while crystallography still wins for small, rigid targets and for very high-resolution ligand work.
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Thousands of individual molecules vitrified in random orientations are imaged and computationally averaged into a 3D Coulomb-potential map, with phases recovered directly from the images so there is no phase problem. It complements crystallography by handling large complexes and membrane proteins that resist crystallization, while crystallography still wins for small, rigid targets and for very high-resolution ligand work.
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