Spinning-Disk Confocal Microscopy
Thousands of pinholes on a rotating disk illuminate and descan many points in parallel onto a camera, yielding hundreds of sectioned frames per second. Parallelization lowers per-molecule excitation dose, but fixed pinhole size and pinhole crosstalk degrade sectioning in thick or densely labelled samples.
This Concept is waiting for its first lesson!
Thousands of pinholes on a rotating disk illuminate and descan many points in parallel onto a camera, yielding hundreds of sectioned frames per second. Parallelization lowers per-molecule excitation dose, but fixed pinhole size and pinhole crosstalk degrade sectioning in thick or densely labelled samples.
Are you a teacher? Sign in to start contributing.
Sign In