Steady-State Approximation
After a brief pre-steady-state burst, d[ES]/dt is taken as zero so ES formation and breakdown balance; setting k1[E][S] = (k-1 + kcat)[ES] and using total enzyme conservation yields a closed-form rate law.
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After a brief pre-steady-state burst, d[ES]/dt is taken as zero so ES formation and breakdown balance; setting k1[E][S] = (k-1 + kcat)[ES] and using total enzyme conservation yields a closed-form rate law.
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