Super-Resolution Microscopy
Techniques that beat the diffraction limit by patterning excitation, by depleting emission at the periphery of the spot, or by localizing sparse single emitters far more precisely than the width of the point spread function. All of them buy resolution with photons, so they demand brighter, more photostable labels and tolerate less live-cell dose than confocal.
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Techniques that beat the diffraction limit by patterning excitation, by depleting emission at the periphery of the spot, or by localizing sparse single emitters far more precisely than the width of the point spread function. All of them buy resolution with photons, so they demand brighter, more photostable labels and tolerate less live-cell dose than confocal.
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