Total Protein Normalization
Normalizing each lane to its total transferred protein, measured by a stain-free gel activation or a whole-lane membrane stain, avoids assuming that a single housekeeping protein is constant across treatments. It also covers a far wider linear range than an abundant housekeeping band, which usually saturates at loads where the target is barely detectable.
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Normalizing each lane to its total transferred protein, measured by a stain-free gel activation or a whole-lane membrane stain, avoids assuming that a single housekeeping protein is constant across treatments. It also covers a far wider linear range than an abundant housekeeping band, which usually saturates at loads where the target is barely detectable.
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