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Transcription

RNA polymerase II binds a promoter, unwinds the duplex and synthesizes a pre-mRNA copy of the template strand 5' to 3'. The rate and frequency of this event across a cell population is what an expression value estimates.

Questions this Concept answers

Why must RNA polymerase II unwind the DNA duplex before it can synthesize RNA?

Demerzel Video

Transcription

RNA polymerase binds a promoter, unwinds the duplex, and synthesises an RNA copy of the template strand 5' to 3'. Both natural crRNA production and expression of an sgRNA from a vector depend on it.