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Transcription

RNA polymerase II binds a promoter, unwinds the duplex and synthesizes a pre-mRNA copy of the template strand 5' to 3'. The rate and frequency of this event across a cell population is what an expression value estimates.

Demerzel Video

Transcription

RNA polymerase binds a promoter, unwinds the duplex, and synthesises an RNA copy of the template strand 5' to 3'. Both natural crRNA production and expression of an sgRNA from a vector depend on it.