Validating Screen Hits with Individual Guides and Orthogonal Assays

Evaluate-level closure for the screen branch.

Questions this Concept answers

Why is a hit identified from a pooled screen considered a candidate rather than a confirmed result?

jeremy Text

Genome Edge

A hit list from a pooled CRISPR screen is a hypothesis, not a result, because off-target cutting, variable guide efficiency and clonal drift all generate false positives. Validation starts by re-test…