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Vapour Diffusion Crystallization

A drop of protein mixed with reservoir solution is equilibrated against a larger reservoir; water leaves the drop as vapour until the two match in chemical potential, slowly concentrating both protein and precipitant. Hanging-drop and sitting-drop geometries trace a defined path across the phase diagram set by the initial drop-to-reservoir dilution.

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A drop of protein mixed with reservoir solution is equilibrated against a larger reservoir; water leaves the drop as vapour until the two match in chemical potential, slowly concentrating both protein and precipitant. Hanging-drop and sitting-drop geometries trace a defined path across the phase diagram set by the initial drop-to-reservoir dilution.

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