Wallace Rule for Melting Temperature
The approximation Tm = 2(A+T) + 4(G+C) in degrees Celsius, valid only for short oligos under standard salt. It ignores stacking context and salt, so it drifts several degrees from measured Tm outside its narrow domain.
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The approximation Tm = 2(A+T) + 4(G+C) in degrees Celsius, valid only for short oligos under standard salt. It ignores stacking context and salt, so it drifts several degrees from measured Tm outside its narrow domain.
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