Western Blot Artifact Diagnosis
Blot failures map to stages: no signal points to failed transfer, dead conjugate, or absent target; uniform high background to insufficient blocking or excess secondary; extra bands to antibody cross-reactivity, degradation, or splice variants; and smiling or frowning bands to Joule heating and uneven current across the gel. Reading the pattern identifies which step to repeat rather than repeating the whole blot.
This Concept is waiting for its first lesson!
Blot failures map to stages: no signal points to failed transfer, dead conjugate, or absent target; uniform high background to insufficient blocking or excess secondary; extra bands to antibody cross-reactivity, degradation, or splice variants; and smiling or frowning bands to Joule heating and uneven current across the gel. Reading the pattern identifies which step to repeat rather than repeating the whole blot.
Are you a teacher? Sign in to start contributing.
Sign In