Z-Stack Acquisition
A sequence of optically sectioned images captured at regular focus increments through the specimen, with step size set by the axial Nyquist criterion rather than by convenience. Refractive index mismatch between immersion medium and sample compresses or stretches the true depth relative to the nominal stage travel.
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A sequence of optically sectioned images captured at regular focus increments through the specimen, with step size set by the axial Nyquist criterion rather than by convenience. Refractive index mismatch between immersion medium and sample compresses or stretches the true depth relative to the nominal stage travel.
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