graph LR idea_028b3282_a89d_4297_9834_1cfb09b6a3bb["Amplification Efficiency from Standard Curve Slope"] idea_0f64d5af_5eb0_49f7_a228_b0d94602ed12["Slope of a Line"] idea_18183526_82bc_4520_b6e1_f3fded7532c4["No-Reverse-Transcriptase Control"] idea_1d88b923_90bb_4a41_ac1d_c62ef9262a1e["Poisson Distribution"] idea_279977ea_6fa6_496b_9f8d_b4aa8b4c0ba7["Technical Versus Biological Replicates"] idea_3124c18a_8ed2_4754_813c_06994c8a6870["Reference Gene Selection and Validation"] idea_33d466e3_49ba_4b84_a7f1_bd33770034b3["Intron-Spanning Primers"] idea_40966119_d8b0_467f_9107_361afca554c4["Geometric Mean"] idea_441d6774_e451_4f6d_8ff7_624a6c618afb["Digital PCR"] idea_46e6d17b_9f5c_4cfe_872f_68f48803ba0a["PCR Primer Design"] idea_494741f4_5a71_4137_9ea6_5d8517fe39f0["Reverse Transcription"] idea_58b1c07a_80f8_4870_a37c_e4eee22e2e06["DNase I Treatment"] idea_5a80f955_6462_45d5_bf19_95697f787eb4["Exponential Growth"] idea_5b6d6952_a4a6_4e7c_8121_41598fbe735b["Dynamic Range and Limit of Detection in qPCR"] idea_5ea8adb9_e2a3_4b3d_a760_93b8310b9f12["Quantification Cycle"] idea_62d1f488_58f8_484c_a59c_3b113bdf944c["TaqMan Hydrolysis Probe"] idea_64d4a757_d878_46a1_a297_bfa7cc131a80["Housekeeping Gene"] idea_6ea9ee32_745b_4d37_85d8_23b023e5e08a["DNA Melting Temperature"] idea_7294a4b1_1c33_46a3_8185_8ce3fe8d1a98["qPCR Amplification Curve Phases"] idea_735d7c3a_05b7_4f7c_9f54_e052f912c11b["SYBR Green I Intercalating Dye"] idea_74615fc7_274e_4c58_9a9d_80abc91663bc["Pseudoreplication"] idea_7a54947b_731e_4f02_b811_a52908944c9d["geNorm M Value"] idea_7d676986_0112_469f_82f8_b2e244a4050f["Fluorescence"] idea_7e20eb72_3f11_4ab3_81d7_a57ad8b58df3["Absolute Versus Relative Quantification"] idea_7f089850_4961_44bf_9e60_6cf4f035e815["Baseline and Threshold Setting in qPCR"] idea_86571229_f678_425c_86bc_e98ff28d6907["Propagation of Uncertainty"] idea_8c911c68_fb56_4e9b_9cb4_a3eee52d8506["Systematic Error"] idea_951054bb_1484_43a7_8478_fffdee86f773["Standard Curve"] idea_98ff7239_ebb1_4232_8fc7_2f2575e46c59["Inter-Run Calibration"] idea_9b283772_d726_4470_9bcd_2bc6b1582426["RNA Integrity Number"] idea_9fec39a4_f73b_413d_ae54_d0cf046b633d["Gene Expression"] idea_a2397421_cf15_4d96_a8d0_00f940ec0c03["Linear Regression"] idea_a5fce989_112a_429e_bb39_61c240d96285["Molar Concentration"] idea_a72e14d1_6f41_4bc0_b9bb_ddbd9be57c55["2^-ddCt Method"] idea_a848fe3f_4641_49c0_b027_a70ba4daf288["Pfaffl Method"] idea_a90dac88_9bf9_45dd_a2a6_b1e289cb5bfb["Exponential Amplification Assumption in qPCR"] idea_ac38ee88_08f8_47d3_bb45_5d8b4545d64f["Melt Curve Analysis"] idea_ad328b02_f377_45f3_87b0_891d377b07b8["Limit of Detection"] idea_bd389d68_4696_474d_945f_539501f5e4a6["qPCR Standard Curve"] idea_c1f1e98b_8f46_4f2e_9839_6f6145ba083b["Nested Experimental Design"] idea_c4beb02b_7eed_40d6_8dc6_5502bbdd5faa["Logarithm"] idea_d3966100_6b40_4f4d_bd16_f4d4f1616e34["Fold Change"] idea_d3ce180e_cc75_47a8_a78b_5d70574b2919["Coefficient of Variation"] idea_df8f9038_7264_4e83_88d3_0564abc91014["No-Template Control and Primer-Dimer Detection"] idea_e50c448e_4db4_47b5_9d22_d3729d82ec25["Polymerase Chain Reaction"] idea_eba2585c_3b79_4052_ac4d_1dd7bcad7402["Serial Dilution"] idea_f2e3385e_d737_4d2c_9cdd_5be18d059970["MIQE Guidelines"] idea_d3966100_6b40_4f4d_bd16_f4d4f1616e34 --> idea_a848fe3f_4641_49c0_b027_a70ba4daf288 idea_c4beb02b_7eed_40d6_8dc6_5502bbdd5faa --> idea_5ea8adb9_e2a3_4b3d_a760_93b8310b9f12 idea_a90dac88_9bf9_45dd_a2a6_b1e289cb5bfb --> idea_a72e14d1_6f41_4bc0_b9bb_ddbd9be57c55 idea_5a80f955_6462_45d5_bf19_95697f787eb4 --> idea_a90dac88_9bf9_45dd_a2a6_b1e289cb5bfb idea_ad328b02_f377_45f3_87b0_891d377b07b8 --> idea_5b6d6952_a4a6_4e7c_8121_41598fbe735b idea_e50c448e_4db4_47b5_9d22_d3729d82ec25 --> idea_7294a4b1_1c33_46a3_8185_8ce3fe8d1a98 idea_8c911c68_fb56_4e9b_9cb4_a3eee52d8506 --> idea_7f089850_4961_44bf_9e60_6cf4f035e815 idea_7294a4b1_1c33_46a3_8185_8ce3fe8d1a98 --> idea_5ea8adb9_e2a3_4b3d_a760_93b8310b9f12 idea_028b3282_a89d_4297_9834_1cfb09b6a3bb --> idea_f2e3385e_d737_4d2c_9cdd_5be18d059970 idea_86571229_f678_425c_86bc_e98ff28d6907 --> idea_a848fe3f_4641_49c0_b027_a70ba4daf288 idea_bd389d68_4696_474d_945f_539501f5e4a6 --> idea_7e20eb72_3f11_4ab3_81d7_a57ad8b58df3 idea_9fec39a4_f73b_413d_ae54_d0cf046b633d --> idea_7e20eb72_3f11_4ab3_81d7_a57ad8b58df3 idea_7294a4b1_1c33_46a3_8185_8ce3fe8d1a98 --> idea_7f089850_4961_44bf_9e60_6cf4f035e815 idea_9fec39a4_f73b_413d_ae54_d0cf046b633d --> idea_3124c18a_8ed2_4754_813c_06994c8a6870 idea_5ea8adb9_e2a3_4b3d_a760_93b8310b9f12 --> idea_bd389d68_4696_474d_945f_539501f5e4a6 idea_279977ea_6fa6_496b_9f8d_b4aa8b4c0ba7 --> idea_f2e3385e_d737_4d2c_9cdd_5be18d059970 idea_7e20eb72_3f11_4ab3_81d7_a57ad8b58df3 --> idea_a72e14d1_6f41_4bc0_b9bb_ddbd9be57c55 idea_c1f1e98b_8f46_4f2e_9839_6f6145ba083b --> idea_279977ea_6fa6_496b_9f8d_b4aa8b4c0ba7 idea_5a80f955_6462_45d5_bf19_95697f787eb4 --> idea_028b3282_a89d_4297_9834_1cfb09b6a3bb idea_3124c18a_8ed2_4754_813c_06994c8a6870 --> idea_f2e3385e_d737_4d2c_9cdd_5be18d059970 idea_98ff7239_ebb1_4232_8fc7_2f2575e46c59 --> idea_f2e3385e_d737_4d2c_9cdd_5be18d059970 idea_46e6d17b_9f5c_4cfe_872f_68f48803ba0a --> idea_df8f9038_7264_4e83_88d3_0564abc91014 idea_028b3282_a89d_4297_9834_1cfb09b6a3bb --> idea_a848fe3f_4641_49c0_b027_a70ba4daf288 idea_1d88b923_90bb_4a41_ac1d_c62ef9262a1e --> idea_5b6d6952_a4a6_4e7c_8121_41598fbe735b idea_74615fc7_274e_4c58_9a9d_80abc91663bc --> idea_279977ea_6fa6_496b_9f8d_b4aa8b4c0ba7 idea_735d7c3a_05b7_4f7c_9f54_e052f912c11b --> idea_df8f9038_7264_4e83_88d3_0564abc91014 idea_7a54947b_731e_4f02_b811_a52908944c9d --> idea_3124c18a_8ed2_4754_813c_06994c8a6870 idea_40966119_d8b0_467f_9107_361afca554c4 --> idea_3124c18a_8ed2_4754_813c_06994c8a6870 idea_c4beb02b_7eed_40d6_8dc6_5502bbdd5faa --> idea_a90dac88_9bf9_45dd_a2a6_b1e289cb5bfb idea_a2397421_cf15_4d96_a8d0_00f940ec0c03 --> idea_bd389d68_4696_474d_945f_539501f5e4a6 idea_64d4a757_d878_46a1_a297_bfa7cc131a80 --> idea_a72e14d1_6f41_4bc0_b9bb_ddbd9be57c55 idea_951054bb_1484_43a7_8478_fffdee86f773 --> idea_bd389d68_4696_474d_945f_539501f5e4a6 idea_9b283772_d726_4470_9bcd_2bc6b1582426 --> idea_f2e3385e_d737_4d2c_9cdd_5be18d059970 idea_bd389d68_4696_474d_945f_539501f5e4a6 --> idea_028b3282_a89d_4297_9834_1cfb09b6a3bb idea_7d676986_0112_469f_82f8_b2e244a4050f --> idea_7294a4b1_1c33_46a3_8185_8ce3fe8d1a98 idea_7d676986_0112_469f_82f8_b2e244a4050f --> idea_7f089850_4961_44bf_9e60_6cf4f035e815 idea_5ea8adb9_e2a3_4b3d_a760_93b8310b9f12 --> idea_a90dac88_9bf9_45dd_a2a6_b1e289cb5bfb idea_33d466e3_49ba_4b84_a7f1_bd33770034b3 --> idea_18183526_82bc_4520_b6e1_f3fded7532c4 idea_c4beb02b_7eed_40d6_8dc6_5502bbdd5faa --> idea_028b3282_a89d_4297_9834_1cfb09b6a3bb idea_6ea9ee32_745b_4d37_85d8_23b023e5e08a --> idea_ac38ee88_08f8_47d3_bb45_5d8b4545d64f idea_494741f4_5a71_4137_9ea6_5d8517fe39f0 --> idea_18183526_82bc_4520_b6e1_f3fded7532c4 idea_441d6774_e451_4f6d_8ff7_624a6c618afb --> idea_7e20eb72_3f11_4ab3_81d7_a57ad8b58df3 idea_ac38ee88_08f8_47d3_bb45_5d8b4545d64f --> idea_df8f9038_7264_4e83_88d3_0564abc91014 idea_58b1c07a_80f8_4870_a37c_e4eee22e2e06 --> idea_18183526_82bc_4520_b6e1_f3fded7532c4 idea_86571229_f678_425c_86bc_e98ff28d6907 --> idea_279977ea_6fa6_496b_9f8d_b4aa8b4c0ba7 idea_64d4a757_d878_46a1_a297_bfa7cc131a80 --> idea_3124c18a_8ed2_4754_813c_06994c8a6870 idea_62d1f488_58f8_484c_a59c_3b113bdf944c --> idea_7294a4b1_1c33_46a3_8185_8ce3fe8d1a98 idea_d3966100_6b40_4f4d_bd16_f4d4f1616e34 --> idea_a72e14d1_6f41_4bc0_b9bb_ddbd9be57c55 idea_7d676986_0112_469f_82f8_b2e244a4050f --> idea_ac38ee88_08f8_47d3_bb45_5d8b4545d64f idea_5a80f955_6462_45d5_bf19_95697f787eb4 --> idea_7294a4b1_1c33_46a3_8185_8ce3fe8d1a98 idea_0f64d5af_5eb0_49f7_a228_b0d94602ed12 --> idea_028b3282_a89d_4297_9834_1cfb09b6a3bb idea_bd389d68_4696_474d_945f_539501f5e4a6 --> idea_5b6d6952_a4a6_4e7c_8121_41598fbe735b idea_a5fce989_112a_429e_bb39_61c240d96285 --> idea_7e20eb72_3f11_4ab3_81d7_a57ad8b58df3 idea_eba2585c_3b79_4052_ac4d_1dd7bcad7402 --> idea_bd389d68_4696_474d_945f_539501f5e4a6 idea_735d7c3a_05b7_4f7c_9f54_e052f912c11b --> idea_ac38ee88_08f8_47d3_bb45_5d8b4545d64f idea_7f089850_4961_44bf_9e60_6cf4f035e815 --> idea_5ea8adb9_e2a3_4b3d_a760_93b8310b9f12 idea_a72e14d1_6f41_4bc0_b9bb_ddbd9be57c55 --> idea_a848fe3f_4641_49c0_b027_a70ba4daf288 idea_d3ce180e_cc75_47a8_a78b_5d70574b2919 --> idea_279977ea_6fa6_496b_9f8d_b4aa8b4c0ba7 style idea_8c911c68_fb56_4e9b_9cb4_a3eee52d8506 fill:#fef3c7,stroke:#f59e0b,color:#92400e style idea_e50c448e_4db4_47b5_9d22_d3729d82ec25 fill:#fef3c7,stroke:#f59e0b,color:#92400e style idea_86571229_f678_425c_86bc_e98ff28d6907 fill:#fef3c7,stroke:#f59e0b,color:#92400e style idea_279977ea_6fa6_496b_9f8d_b4aa8b4c0ba7 fill:#e5e7eb,stroke:#9ca3af,color:#374151 style idea_46e6d17b_9f5c_4cfe_872f_68f48803ba0a fill:#fef3c7,stroke:#f59e0b,color:#92400e style 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