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About CRISPR Gene Editing: From Bacterial Immunity to Genome Surgery

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Trace CRISPR from a bacterial adaptive-immune system to the clinical genome editors used today. You will learn how Cas9 finds a target through PAM recognition and guide-RNA base pairing, what the cell actually does with the resulting break (NHEJ indels versus HDR knock-in), and why off-target editing drove the shift to high-fidelity nucleases and break-free editors. The path finishes with base editing, prime editing, LNP and AAV delivery, and the approved therapies that have shipped.

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What You'll Learn

Concepts:
Protospacer Adjacent Motif Gene Knockout by Non-Homologous End Joining Cas9 Nuclease Mechanism Guide RNA Design High-Fidelity Cas9 Variants Double-Strand Break Repair Pathway Choice AAV Delivery of Gene Editors CRISPR-Cas Adaptive Immunity Lipid Nanoparticle Delivery of Gene Editors Approved CRISPR Therapies Human Germline Editing Ethics Prime Editing Base Editing CRISPR Off-Target Effects CRISPR Editing Platform Trade-Offs Knock-In by Homology-Directed Repair

What you will learn

CRISPR Gene Editing: From Bacterial Immunity to Genome Surgery

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