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About Reading Mass Spectra: From m/z Peaks to Molecular Structure

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You will learn to take a raw mass spectrum and work it back to a molecule. Starting from what the x-axis actually measures, you will identify the molecular ion, count carbons and halogens from isotope patterns, apply the nitrogen rule and rings-plus-double-bonds to constrain formulas, and rationalize fragments through alpha cleavage, McLafferty rearrangement, and characteristic neutral losses. You will then interpret accurate-mass data to assign a molecular formula, read multiply charged electrospray envelopes and deconvolute them, and interpret MS/MS product-ion spectra from GC-MS and LC-MS runs where retention time is a second dimension of evidence.

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What You'll Learn

Concepts:
Nitrogen Rule Molecular Formula Determination from Accurate Mass Gas Chromatography-Mass Spectrometry Charge State Deconvolution Neutral Loss Fragmentation M+1 Peak Carbon Counting Liquid Chromatography-Mass Spectrometry Tandem Mass Spectrometry Multiply Charged Ions Mass-to-Charge Ratio Isotope Patterns in Mass Spectra Hard Versus Soft Ionization Molecular Ion Peak Alpha Cleavage McLafferty Rearrangement Rings Plus Double Bonds Equivalent

What you will learn

No introduction video available

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